Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /

• Main Authors: Till, Bradley J. (Author), Jankowicz-Cieslak, Joanna (Author), Huynh, Owen A. (Author), Beshir, Mayada M. (Author), Laport, Robert G. (Author), Hofinger, Bernhard J. (Author)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: Cham : Springer International Publishing : Imprint: Springer, 2015.
• Subjects: Life sciences.; Nucleic acids.; Plant breeding.; Biology > Technique.; Life Sciences.; Plant Breeding/Biotechnology.; Biological Techniques.; Nucleic Acid Chemistry.

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single...