Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /

• Κύριοι συγγραφείς: Till, Bradley J. (Συγγραφέας), Jankowicz-Cieslak, Joanna (Συγγραφέας), Huynh, Owen A. (Συγγραφέας), Beshir, Mayada M. (Συγγραφέας), Laport, Robert G. (Συγγραφέας), Hofinger, Bernhard J. (Συγγραφέας)
• Συγγραφή απο Οργανισμό/Αρχή: SpringerLink (Online service)
• Μορφή: Ηλεκτρονική πηγή Ηλ. βιβλίο
• Γλώσσα: English
• Έκδοση: Cham : Springer International Publishing : Imprint: Springer, 2015.
• Θέματα: Life sciences.; Nucleic acids.; Plant breeding.; Biology > Technique.; Life Sciences.; Plant Breeding/Biotechnology.; Biological Techniques.; Nucleic Acid Chemistry.

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single...

Πίνακας περιεχομένων:

• Introduction
• Health and Safety Considerations
• Sample Collection and Storage
• Low-Cost DNA Extraction
• PCR Amplification for Low-Cost Mutation Discovery
• Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples
• Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants
• Example Data
• Conclusions.  .