Evaluating and optimizing loading methods of single-chain variable fragment protein into extracellular vesicles

• Main Author: Mohamed, Khaled
• Other Authors: Κλεπετσάνης, Παύλος
• Format: Thesis
• Language: English
• Published: 2020
• Subjects: Nanosized extracellular vesicles; Protein transfer; FDA; Νανοσωματιδιακά εξωκυτταρικά κυστίδια; Μεταφορά πρωτεϊνών

by the FDA. While commercially available proteins have an extracellular activity, intracellular targets could be a powerful alternative to treat many diseases (cancer, inflammatory disorders,...). Nevertheless, proteins face many barriers to reach their intracellular targets (internalisation, endoso...