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oapen-20.500.12657-315372021-11-12T16:22:26Z Chapter 14 Coupled Enzyme Activity and Thermal Shift Screening of the Maybridge Rule of 3 Fragment Library Against Trypanosoma brucei Choline Kinase; A Genetically Validated Drug Target Denton, Helen Major, Louise L. Smith, Terry K. Major, Louise L. Denton, Helen Smith, Terry K. pharmacology toxicology pharmacology toxicology Assay Enzyme Molar concentration Protein Thermal shift assay Trypanosoma brucei bic Book Industry Communication::M Medicine::MM Other branches of medicine::MMG Pharmacology In this study we interrogate 630 compounds of the Maybridge Rule of 3 Fragment Library for compounds that interact with, and inhibit TbCK. The Maybridge Rule of 3 Fragment Library is a small collection of quantifiable diverse, pharmacophoric rich, chemical entities that comply with the following criteria; MW ≤ 300, cLogP ≤ 3, H-Bond Acceptors ≤ 3, H-Bond Donors ≤ 3, Rotatable bonds (Flexibility Index) ≤ 3, Polar Surface Area ≤ 60 Å2 and aqueous solubility ≥ 1 mM using LogS and high purity (≥ 95%). Comparisons between two different screening methods, a coupled enzyme activity assay and differential scanning fluorimetry, has allowed identification of compounds that interact and inhibit the T. brucei choline kinase, several of which possess selective trypanocidal activity. Screening of a comparatively small fragment library by two different screening methods has allowed identification of several compounds that interact with and inhibit TbCK, a genetically validated drug target against African sleeping sickness. Some of the inhibitory fragments were also selectively trypanocidal, considering these are relatively simple molecules with no optimization, finding low μΜ inhibitors is very encouraging. Moreover some of the morphological phenotypes of these trypanocidal compounds include cell-cycle arrests similar to those observed for the TbCK conditional knockout grown under permissive conditions. 2019-10-04 14:47:26 2020-04-01T13:38:56Z 2017-04-12 23:55 2019-10-04 14:47:26 2020-04-01T13:38:56Z 2017-03-01 23:55:55 2019-10-04 14:47:26 2020-04-01T13:38:56Z 2020-04-01T13:38:56Z 2013 chapter 627366 OCN: 1030819386 http://library.oapen.org/handle/20.500.12657/31537 eng application/pdf n/a 627366.pdf InTechOpen Drug Discovery 10.5772/52668 10.5772/52668 09f6769d-48ed-467d-b150-4cf2680656a1 11e5898d-b0b7-4d63-a871-e2c2be6443ad d859fbd3-d884-4090-a0ec-baf821c9abfd Wellcome 1 067441 Wellcome Trust Wellcome open access
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In this study we interrogate 630 compounds of the Maybridge Rule of 3 Fragment Library for compounds that interact with, and inhibit TbCK. The Maybridge Rule of 3 Fragment Library is a small collection of quantifiable diverse, pharmacophoric rich, chemical entities that comply with the following criteria; MW ≤ 300, cLogP ≤ 3, H-Bond Acceptors ≤ 3, H-Bond Donors ≤ 3, Rotatable bonds (Flexibility Index) ≤ 3, Polar Surface Area ≤ 60 Å2 and aqueous solubility ≥ 1 mM using LogS and high purity (≥ 95%). Comparisons between two different screening methods, a coupled enzyme activity assay and differential scanning fluorimetry, has allowed identification of compounds that interact and inhibit the T. brucei choline kinase, several of which possess selective trypanocidal activity. Screening of a comparatively small fragment library by two different screening methods has allowed identification of several compounds that interact with and inhibit TbCK, a genetically validated drug target against African sleeping sickness. Some of the inhibitory fragments were also selectively trypanocidal, considering these are relatively simple molecules with no optimization, finding low μΜ inhibitors is very encouraging. Moreover some of the morphological phenotypes of these trypanocidal compounds include cell-cycle arrests similar to those observed for the TbCK conditional knockout grown under permissive conditions.
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