Insect histology : practical laboratory techniques /

• Main Author: Barbosa, Pedro, 1944-
• Other Authors: Berry, Deborah L., 1972-, Kary, Christina S., 1975-
• Format: eBook
• Language: English
• Published: Chichester, West Sussex ; Hoboken, NJ : Wiley/Blackwell, 2014.
• Edition: Second edition.
• Subjects: Entomology > Laboratory manuals.; Histology > Laboratory manuals.; Entomology.; Histology.; SCIENCE / Life Sciences / Zoology / General; Laboratory manuals.; Electronic books.

This title is a much needed update of Barbosa's self-published Manual of Basic Techniques in Insect Histology. It is a laboratory manual of 'traditional' and 'modern' insect histology techniques, completely revised using cutting-edge methodology carried out today and include...

Table of Contents:

• Problems of sclerotized chitin : softening insect cuticle
• Fixation
• Dehydration, clearing, and embedding
• Staining
• Immunohistochemical staining
• Use of genetic markers in insect histology
• Fluorescence
• Mounting
• Preparation of whole mounts
• Whole mount staining
• Preparation of insect tissues
• Preparation of genitalia, mouthparts, and other body parts
• Preparation of chromosomes
• Appendix : dissecting fluids and insect saline solutions.